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KrosFlo® Perfusion Flow-Path Assemblies


Single-Use Tangential Flow Filtration



  • Ready to Use, Single Use and Sterile
  • Low shear magnetic levitating pump
  • Scalable from 2L to 2000L
  • mPES Hollow fiber filters-low protein binding and high mass transfer
  • Simple and fast set-up
  • Non-invasive flow-path with in-line pressure sensors

Spectrum's TFF assemblies for bioreactor perfusion are ready to use, single-use and gamma sterilized. With the elimination of autoclaving setup time is reduced to minutes from hours compared to existing perfusion technologies. The perfusion flow paths are scalable from 2L to 2000L working volumes. The patent pending design incorporates a low shear-levitating magnetic pump that is single-useand is sterilized with the complete assembly. These single-use pumps reduce the risk of contamination due to their self-contained design. Unlike peristaltic pumps, they are pulsation free and low shear with no particle generation and no impact on cell viability based upon process configuration within each system.

The assemblies also include Spectrum's hollow fiber filters with the advanced modified polyethersulfone (mPES) membrane chemistry. mPES hollow fiber filters provide high mass transfer with low protein binding. The hollow fiber filters used in the assemblies are available with 0.5 mm or 1.0 mm lumens and with pore sizes for both microfiltration and ultrafiltration. The perfusion assemblies can be used to achieve cell counts over 100 x  106 cells/ml. The KrosFlo assemblies also include flexible tubing and inline polysulfone pressure transmitters for monitoring feed, retentate and permeate pressures.

The novel design of these disposable sterile assemblies with the single-use pump, hollow fiber filter, in-line sensors, tubing and pressure transmitters give excellent perfusion results with minimal setup time. If necessary they can be replaced during operation. The KrosFlo Perfusion Assembly designs can be used in either scale up or scale down applications. They can be used with either disposable or reusable bioreactors with capacities ranging from 2L to 2000L. The 200 series low shear-levitating pump is designed to operate below the limits of its maximum pressure differential of 2.6 bar (37.7 psi) and maximum flow rate to 21 LPM. The operation conditions for the KPS 200 are based upon flow rates less than 4 LPM at a pressure drop of less than one bar (14.5 psi). The 600 series pump is designed for a maximum pressure differential or 3.2 bar (46.4 psi) and maximum flow rate of 75 LPM. The operating conditions for the KPS 600 are based upon flow rates less than 45 LPM at a pressure drop of less than one bar (14.5 psi).

KrosFlo Perfusion System ( KML 100 and KPS)
Sterile Disposable Perfusion Flow-Path
0.5mm Lumen ID Hollow Fiber
Bioreactor Size
(Liter)
Module
Area (m2)
Recirculation
(LPM)
System Retentate Shear
Rate Range
(Sec-1)
Suggested Module
Series
2 to 5 0.08 0.1 - 0.38 KML 100 / KPS 200 500 - 2000 Sampler /S02-
10 to 25 0.26 - 0.31 0.32 - 1.55 KML 100 / KPS 200 500 - 2000 MiniKros® /N02-
30 to 50 0.54 - 0.65 0.32 - 1.55 KML 100 / KPS 200 500 - 2000 MiniKros® /N04-
60 to 100 0.85 - 1.0 0.32 - 1.55 KML 100 / KPS 200 500 - 2000 MiniKros® /N06-
125 to 200 1.25 - 1.6 1.5 - 6 KPS 600 500 - 2000 KrosFlo® /K02-
250 to 500 2.6 - 3.25 1.5 - 6 KPS 600 500 - 2000 KrosFlo® /K04-
750 to 1000 9.5 - 12.5 4.6 - 18.5 KPS 600 500 - 2000 KrosFlo® Max /X04-
2000 9.5 - 12.5 (x) 2 9.2 - 37 KPS 600 500 - 2000 KrosFlo® Max /X06-
0.63 - 1.0 mm Lumen ID Hollow Fiber

Bioreactor Size
(Liter)
Module
Area (m2)
Recirculation
(LPM)
System Retentate Shear
Rate Range
(Sec-1)
Suggested Module
Series
2 to 5 0.047 - 0.053 0.23 - 0.95 KML 100 / KPS 200 500 - 2000 Sampler /S02-
10 to 25 0.31 - 0.33 0.75 - 3.0 KML 100 / KPS 200 500 - 2000 MiniKros® /N04-
30 to 50 0.48 - 0.51 0.75 - 3.0 KML 100 / KPS 200 500 - 2000 MiniKros® /N06-
60 to 100 0.78 - 1.0 3.7 - 14.8 KPS 600 500 - 2000 KrosFlo® /K02-
125 to 200 1.55 - 1.65 3.7 - 14.8 KPS 600 500 - 2000 KrosFlo® /K04-
250 to 500 2.43 - 2.55 3.7 - 14.8 KPS 600 500 - 2000 KrosFlo® /K06-
750 to 1000 5.2 - 7.1 9.9 - 40 KPS 600 500 - 2000 KrosFlo® Max /X04-
2000 5.2 - 7.1 (x) 2 9.9 - 40 (x) 2 KPS 600 500 - 2000 KrosFlo® Max /X06-
for more information:

call Customer Service at (800) 634-3300 (toll-free US and Canada) • (310) 885-4600 (world-wide)

email us at customerservice@spectrumlabs.com

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hi dave
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